Information for users:
The Facility is available to Midwest researchers. Potential users can learn more about our system by viewing the seminar below, “The ISU Confocal Microscopy Core Facility: overview and applications for biological research.”
Part 1. Basic overview of the Leica SP8 Confocal system; lightpath; White Light Laser; Synergy among components. 15 min.
Part 2. Spectral fingerprinting with Ex/Em plots; Fluorescence Lifetime Imaging Microscopy (FLIM). 13 min.
Part 3. Lightning deconvolution with an example using endosomes; use of Zoom conferencing on the confocal. 18 min.
Part 4. This video provides an overview of the confocal lightpath and covers details of the excitation side: White light laser (WLL) mechanism; AOTF control of WLL; The laser array; Scanner options.