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Information for users:

The Facility is available to Midwest researchers. Potential users can learn more about our system by viewing the seminar below, “The ISU Confocal Microscopy Core Facility: overview and applications for biological research.”

Part 1. Basic overview of the Leica SP8 Confocal system; lightpath; White Light Laser; Synergy among components. 15 min.

Part 2. Spectral fingerprinting with Ex/Em plots; Fluorescence Lifetime Imaging Microscopy (FLIM). 13 min.

Part 3. Lightning deconvolution with an example using endosomes; use of Zoom conferencing on the confocal. 18 min.

Part 4. This video provides an overview of the confocal lightpath and covers details of the excitation side: White light laser (WLL) mechanism; AOTF control of WLL; The laser array; Scanner options.

Video: Multiple Imaging Modes for Enhanced Analysis of Drosophila Cells and Tissues with the Leica SP8 Confocal Microscope System (2022 Poster Presentation). Discusses new examples of spectral analysis, FLIM, and resolution enhancement with Lightning deconvolution.

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