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ISU Advanced Bioimaging Facility

Leica SP8 Confocal Microscope

The ISU Advanced Bioimaging Facility is open to users from throughout the Midwest region. The Facility features a 2019 Leica SP8 Confocal Microscope with White Light Laser and Fluorescence Lifetime Imaging Microscopy (FLIM) capability. The SP8 is funded by a grant from the National Science Foundation. See the award page for more details.

See the Videos page for a discussion of the facility and some of the available applications.

For scheduling and user information please contact Kevin Edwards, kaedwar@ilstu.edu

Training courses: The graduate training course for the SP8 is BSC 411 Confocal Microscopy in Biology. It will be offered in Fall 2022. Students: BSC 411 has 1 hr per week of lecture/computer work from 1-1:50 on Fridays, and a separate 75 min of training per week at the SP8. The lab section schedule will be determined once we know everyone’s availability. There is limited space, so please email kaedwar@ilstu.edu if you plan to enroll. Undergraduates: BSC 356 is the undergrad SP8 training course, and runs concurrently with BSC 411; contact Dr. Edwards for an override.  

Confocal microscope
ISU Leica SP8 Confocal Microscope
Multichannel imaging with Leica SP8 Confocal Microscope
Microscope at ISU
ISU Leica SP8 Confocal System: DMi8 Microscope
Leica software screenshot
ISU Confocal Microscope: Multichannel imaging with Leica LAS X software
Schematic diagram of confocal microscope
ISU Leica SP8 Confocal Microscope: schematic diagram of light path
Microscope images comparing confocal and widefield microscopy
Left, confocal and right, widefield microscopy images of an insect ovary stained with f-actin stain, DAPI, and wheat germ agglutinin.
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