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ISU Advanced Bioimaging Facility

Leica SP8 Confocal Microscope

The ISU Advanced Bioimaging Facility is open to users from throughout the Midwest region. The Facility features a 2019 Leica SP8 Confocal Microscope with White Light Laser and Fluorescence Lifetime Imaging Microscopy (FLIM) capability. The SP8 is funded by a grant from the National Science Foundation. See the award page for more details.

See the Videos page for a discussion of the facility and some of the available applications.

For scheduling and user information please contact Kevin Edwards,

Training courses: The graduate training course for the SP8 is BSC 411 Confocal Microscopy in Biology. It will be offered in Fall 2023. Students: BSC 411 has 1 hr per week of lecture/computer work from 1-1:50 on Fridays, and a separate 75 min of training per week at the SP8. The lab section schedule will be determined once we know everyone’s availability. There is limited space, so please email if you plan to enroll. Undergraduates: BSC 356 is the undergrad SP8 training course, and runs concurrently with BSC 411; contact Dr. Edwards for an override.  

Confocal microscope
ISU Leica SP8 Confocal Microscope
Multichannel imaging with Leica SP8 Confocal Microscope
Microscope at ISU
ISU Leica SP8 Confocal System: DMi8 Microscope
Leica software screenshot
ISU Confocal Microscope: Multichannel imaging with Leica LAS X software
Schematic diagram of confocal microscope
ISU Leica SP8 Confocal Microscope: schematic diagram of light path
Microscope images comparing confocal and widefield microscopy
Left, confocal and right, widefield microscopy images of an insect ovary stained with f-actin stain, DAPI, and wheat germ agglutinin.
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